Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive HF
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Suspension Culture
SUBMITTER: Tamar Ziv
LAB HEAD: Jonathan Leor
PROVIDER: PXD033253 | Pride | 2023-05-10
REPOSITORIES: Pride
Schary Yeshai Y Rotem Itai I Caller Tal T Lewis Nir N Shaihov-Teper Olga O Brzezinski Rafael Y RY Lendengolts Daria D Raanani Ehud E Sternik Leonid L Naftali-Shani Nili N Leor Jonathan J
Scientific reports 20230318 1
Inflammation and fibrosis limit the reparative properties of human mesenchymal stromal cells (hMSCs). We hypothesized that disrupting the toll-like receptor 4 (TLR4) gene would switch hMSCs toward a reparative phenotype and improve the outcome of cell therapy for infarct repair. We developed and optimized an improved electroporation protocol for CRISPR-Cas9 gene editing. This protocol achieved a 68% success rate when applied to isolated hMSCs from the heart and epicardial fat of patients with is ...[more]