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Proteomic analysis of Blastocrithidia nonstop

ABSTRACT: Proteomic analysis was performed to confirm the identity of in-frame stop codons

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Blastocrithidia Sp. P57

SUBMITTER: David Potesil

LAB HEAD: Zbynek Zdrahal

PROVIDER: PXD033324 | Pride | 2022-12-12

REPOSITORIES: Pride

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Short tRNA anticodon stem and mutant eRF1 allow stop codon reassignment.

Kachale Ambar A Pavlíková Zuzana Z Nenarokova Anna A Roithová Adriana A Durante Ignacio M IM Miletínová Petra P Záhonová Kristína K Nenarokov Serafim S Votýpka Jan J Horáková Eva E Ross Robert L RL Yurchenko Vyacheslav V Beznosková Petra P Paris Zdeněk Z Valášek Leoš Shivaya LS Lukeš Julius J

Nature 20230111 7945

Cognate tRNAs deliver specific amino acids to translating ribosomes according to the standard genetic code, and three codons with no cognate tRNAs serve as stop codons. Some protists have reassigned all stop codons as sense codons, neglecting this fundamental principle<sup>1-4</sup>. Here we analyse the in-frame stop codons in 7,259 predicted protein-coding genes of a previously undescribed trypanosomatid, Blastocrithidia nonstop. We reveal that in this species in-frame stop codons are underrepr ...[more]

PMID: 36631608

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