Dataset Information

Murine IgG (polyclonal) from Listeria Challenged pregnant dams

ABSTRACT: IgG was collected from virgin and pregnant listeria challenged mouse plasma. Polyclonal IgG was analyzed by reduction, alkylation, and LC-MS with top-down MS/MS (HCD and ETD)to look for glycosylation PTMs

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Blood Plasma

SUBMITTER: Stephanie Archer-Hartmann

LAB HEAD: Dr. Parastoo Azadi

PROVIDER: PXD033357 | Pride | 2022-06-28

REPOSITORIES: Pride

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Publications

Pregnancy enables antibody protection against intracellular infection.

Erickson John J JJ Archer-Hartmann Stephanie S Yarawsky Alexander E AE Miller Jeanette L C JLC Seveau Stephanie S Shao Tzu-Yu TY Severance Ashley L AL Miller-Handley Hilary H Wu Yuehong Y Pham Giang G Wasik Brian R BR Parrish Colin R CR Hu Yueh-Chiang YC Lau Joseph T Y JTY Azadi Parastoo P Herr Andrew B AB Way Sing Sing SS

Nature 20220608 7915

Adaptive immune components are thought to exert non-overlapping roles in antimicrobial host defence, with antibodies targeting pathogens in the extracellular environment and T cells eliminating infection inside cells<sup>1,2</sup>. Reliance on antibodies for vertically transferred immunity from mothers to babies may explain neonatal susceptibility to intracellular infections<sup>3,4</sup>. Here we show that pregnancy-induced post-translational antibody modification enables protection against the ...[more]

PMID: 35676476

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Project description:Changes in IgG glycosylation have been reported to be associated with colorectal cancer (CRC), but the alteration in specific subclass of IgG is unknown. Herein, we optimized five common IgG glycopeptides enrichment methods to acquire comprehensive profiling of IgG glycoproteomics in CRC. Notably, incomplete tryptic digestion of IgG occurred when using ordinary ratio of protease to protein, which significantly impacted the final statistical analysis of association between IgG glycosylation and CRC. We introduced a method based on two-step enzymatic digestion, enabling the complete digestion of IgG glycopeptides and further improving the detection intensity of the target glycopeptides. By this approach, total 47 IgG glycopeptides can be identified by nanoLC-ESI-MS/MS. Following rapid and automatic data processing through the self-developed program, we observed that IgG1_H3N4F1 and IgG1_H3N4 were substantially increased in CRC, while IgG1_H5N5F1, IgG1_H5N4F1S1and IgG2_H5N4F1 were markedly decreased. Strikingly, these four glycopeptides were continually significantly changed in early and late stages of CRC. Further evaluation of the diagnostic performance showed they all obtained a fair performance in discriminating the patients from the normal. In terms of the glycan features, it demonstrated that CRC progression associates with the increased agalactosylation, and the decreased digalactosylation and galactosylation per antenna on diantennay glycans of IgG1 and IgG2. Concurrently, the decreased sialylation of IgG1 was strongly correlated with CRC. Moreover, tumor-specific glycosylation analysis showed that the alterations of subclass-specific IgG glycosylation were more significant in colon cancer, and no obvious difference between colon and rectal cancer was found. More importantly, all these findings were validated in an independent cohort. This two-step enzymatic approach is crucial to explore the relationship between the disease progression and IgG glycosylation.

Dataset Information

Murine IgG (polyclonal) from Listeria Challenged pregnant dams

Publications

Pregnancy enables antibody protection against intracellular infection.

Similar Datasets

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