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Heat stressed equine Mesenchymal Stem Cells

ABSTRACT: The aim of this research was to determine the impact of heat stress on cell differentiation in an equine stem cell model through the outcome of heat stress on the proteome. Proteomic analysis was performed using mass spectrometry to compare relative protein abundances among three cell types, mesenchymal stem cells (MSCs), osteoblasts, and adipocytes, cultured at two temperatures, 37°C and 42°C.

INSTRUMENT(S): LTQ Orbitrap Velos Pro

ORGANISM(S): Equus Caballus (horse)

TISSUE(S): Mesenchymal Stem Cell, Stem Cell

SUBMITTER: Ahmad Abd-El-Aziz

LAB HEAD: William Montelpare

PROVIDER: PXD033414 | Pride | 2022-08-12

REPOSITORIES: Pride

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Heat Shock Alters the Proteomic Profile of Equine Mesenchymal Stem Cells.

Abd-El-Aziz Ahmad A Riveroll Angela A Esparza-Gonsalez Blanca B McDuffee Laurie L Cohen Alejandro M AM Fenech Adam L AL Montelpare William J WJ

International journal of molecular sciences 20220629 13

The aim of this research was to determine the impact of heat stress on cell differentiation in an equine mesenchymal stem cell model (EMSC) through the application of heat stress to primary EMSCs as they progressed through the cell specialization process. A proteomic analysis was performed using mass spectrometry to compare relative protein abundances among the proteomes of three cell types: progenitor EMSCs and differentiated osteoblasts and adipocytes, maintained at 37 °C and 42 °C during the ...[more]

PMID: 35806237

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