Dataset Information

Comparative Proteomic Analysis of Seminal Plasma Exosomes in Buffalo with High and Low Sperm Motility

ABSTRACT: Exosomes are nanosized membranous vesicles secreted by variety types of cells and regulate intercellular communication through deliver bioactive substances. They are found in abundance in biological fluids including semen. Although protein composition and potential fertility biomarkers of seminal plasma exosomes (SPE) have been identified in human, but they remains unclear in buffalo. Here, we aim to discover the protein correlation between spermatozoa, seminal plasma (SP) and SPE, then further compare and analyze the protein differences between high and low motility SPEs in buffalo. SPEs were concentrated and purified by ultracentrifugation combined by sucrose density gradient centrifugation, followed by verification by western blot, nanoparticle tracking analysis, and transmission electron microscopy. Protein composition in spermatozoa, SP and SPE, and protein difference in high and low motility SPEs were identified by LC-MS/MS proteomic analysis and functional analyzed by a comprehensive bioinformatic analysis.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Bubalus Bubalis

TISSUE(S): Testis, Sperm

SUBMITTER: qiang Fu

LAB HEAD: Qiang Fu

PROVIDER: PXD033442 | Pride | 2022-12-05

REPOSITORIES: Pride

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Publications

Proteomic profiles of buffalo spermatozoa and seminal plasma.

Fu Qiang Q Pan Limei L Huang Delun D Wang Zhiqiang Z Hou Zhen Z Zhang Ming M

Theriogenology 20190515

A comprehensive identification of the proteins of spermatozoa and seminal plasma enables the full characterization of sperm biology and is essential to understand how seminal plasma influences sperm fertility. In this study, proteomics of buffalo spermatozoa and seminal plasma were analyzed using a bottom-up approach. A total of 2147 and 864 proteins were identified from mature spermatozoa and seminal plasma, respectively. Of these, 371 proteins-42.9% in the seminal plasma set-were common to bot ...[more]

PMID: 31146187

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Project description:Male factors account for approximately 40% of all infertility. Conventional semen analysis focus on seminal physicochemical property and spermatozoa morphology. They yield no information concerning the functional competence of the spermatozoa(Petrunkina et al., 2007). Owning to the essential role of proteins in the reproductive process, comprehensive and systematic identification of proteome is critical to gain new insights into spermatogenesis and infertility. Recent advances in mass spectrometry have allowed the identification of hundreds to thousands of protein in spermatozoa(Oliva et al., 2008; Amaral et al., 2014; Codina et al., 2015). In major domestic mammalian species, global spermatozoa proteomic profiles have been described in rodents(Baker et al., 2008a; Baker et al., 2008b) and bovine(Peddinti et al., 2008). Meanwhile, lacking of new protein synthesis in spermatozoa supports the current view that the regulation of sperm maturation is controlled by exogenous proteins(O'Rand et al., 2011) (e.g., during epididymal transit). Among the seminal plasma proteins, The human seminal plasma has been comprehensively described (Pilch and Mann, 2006; Batruch et al., 2011; Milardi et al., 2012; Milardi et al., 2013; Sharma et al., 2013). In domestic animal species, some studies performing a systematic analysis on using high throughput proteomics have been performed(Kelly et al., 2006; Moura et al., 2007; Souza et al., 2012; Druart et al., 2013; Soleilhavoup et al., 2014). Buffalos (Bubalus bubalis) are adapted to hot–humid tropical climatic conditions, but have low reproductive efficiency. The low sperm motility maybe contributed to protein components variation of spermatozoa and seminal plasma. The composition of buffalo spermatozoa and buffalo seminal plasma (BSP) remains unknown. Proteomic study would be beneficial to the elucidation of the roles of sperm and BSP proteins in regulation of maturation, motility and fertilization. As such, the aim of the current study was to extensively characterize the differentially expressed protein of buffalo spermatozoa and seminal plasma using a comparative proteomics.

Dataset Information

Comparative Proteomic Analysis of Seminal Plasma Exosomes in Buffalo with High and Low Sperm Motility

Publications

Proteomic profiles of buffalo spermatozoa and seminal plasma.

Similar Datasets

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