Proteomics

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Proteome of choline-grown Eubacterium limosum ATCC 8486

ABSTRACT: Eubacterium limosum ATCC 8486 makes acetate and butyrate from various substrates and is found in the human intestine. The proteome of choline -grown Eubacterium limosum was obtained in order to identify enzymes required for growth on choline, in particular to identify components that are unique to growth on choline in comparison to other substrates for acetogenesis, such as lactic acid, L-carnitine, or proline betaine. Choline is converted to trimethylamine (TMA) by certain members of the gut microbiome. Subsequent liver metabolism of TMA is now tied to progression of cardiovascular disease. Demethylation of choline is observed during growth of Eubacterium limosum on this substrate, and does not produce TMA. Choline demethylation by organisms like Eubacterium limosum could lessen TMA production in the gut, thereby lessening the propensity towards atherosclerosis caused by metabolism of TMA in the body. This proteome led to discovery of a phosphocholine:tetrahydrofolate methyltransferase system. The key choline demethylating enzyme is a member of the widespread TMA methyltransferase protein superfamily.

INSTRUMENT(S):

ORGANISM(S): Bacteria Eubacterium Limosum

SUBMITTER: Joseph Krzycki  

LAB HEAD: Joseph Adrian Krzycki

PROVIDER: PXD033467 | Pride | 2025-06-16

REPOSITORIES: Pride

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Dataset's files

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Action DRS
32074_Choline_1_6uG_FRACTION_0.raw Raw
32074_Choline_1_6uG_FRACTION_1.raw Raw
32074_Choline_1_6uG_FRACTION_2.raw Raw
32074_Choline_1_6uG_FRACTION_3.raw Raw
32074_Choline_1_6uG_FRACTION_4.raw Raw
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Publications

A cobalamin-dependent pathway of choline demethylation from the human gut acetogen Eubacterium limosum.

Jiang Ruisheng R   Kountz Duncan J DJ   Zhang Liwen L   Krzycki Joseph A JA  

The Journal of biological chemistry 20250423 6

Elevated serum levels of trimethylamine N-oxide (TMAO) are reported to promote the development of atherosclerosis. TMAO is produced by hepatic oxidation of trimethylamine (TMA) produced by the gut microbiome from dietary quaternary amines such as choline. Net TMA production in the gut depends on microbial enzymes that either produce or consume TMA and its precursors. Here we report the elucidation of a novel microbial pathway consuming choline without TMA production. The human gut acetogen Eubac  ...[more]

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