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Allergen Shedding in Human Milk from Mothers of Preterm Infants: Proteomic and Peptidomic Feasibility and Pilot Analysis

ABSTRACT: There is little information regarding the allergen content of milk feeds in the preterm population. Previous studies have evaluated specific proteins/peptides via ELISA, but no studies have performed a broad analysis of the allergenic peptide content and protease activity of milk feeds in this population. Preterm infants spend a critical window of time for immune development in the Newborn Intensive Care Unit (NICU), and may receive fortified donor milk, maternal milk or formula feeds via nasogastric tube or bottle instead of fresh breastmilk via breastfeeding.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human) Bos Taurus (bovine)

TISSUE(S): Milk

SUBMITTER: Majid Ghassemian

LAB HEAD: Sydney Leibel

PROVIDER: PXD033473 | Pride | 2024-01-26

REPOSITORIES: Pride

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Allergen Content and Protease Activity in Milk Feeds from Mothers of Preterm Infants.

Luskin Kathleen K Mortazavi Diba D Bai-Tong Sherry S Bertrand Kerri K Chambers Christina C Schulkers-Escalante Keriann K Ahmad Alla A Luedtke Stephanie S O'Donoghue Anthony J AJ Ghassemian Majid M Geng Bob B Leibel Sandra L SL Leibel Sydney A SA

Breastfeeding medicine : the official journal of the Academy of Breastfeeding Medicine 20221012 11

Rationale: There is little information regarding the allergen content of milk feeds in the preterm population. Previous studies have not performed a broad analysis of the allergenic peptide content and protease activity of milk feeds in this population. Methods: To evaluate feasibility, we initially performed mass spectrometry on 4 human milk (HM) samples (2 term and 2 preterm) from the Mommy's Milk Human Milk Biorepository (HMB) and analyzed the results against the U ...[more]

PMID: 36251466

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