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Phosphorylation status of histone H1 in mouse embryonic stem cells

ABSTRACT: The aim was to characterize PTMs on histone H1 variants in embryonic stem cells by mass spectrometry.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Embryonic Stem Cell

SUBMITTER: Gabriela Lochmanova

LAB HEAD: Zbynek Zdrahal

PROVIDER: PXD033544 | Pride | 2022-08-12

REPOSITORIES: Pride

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Publications

The Highest Density of Phosphorylated Histone H1 Appeared in Prophase and Prometaphase in Parallel with Reduced H3K9me3, and HDAC1 Depletion Increased H1.2/H1.3 and H1.4 Serine 38 Phosphorylation.

Legartová Soňa S Lochmanová Gabriela G Bártová Eva E

Life (Basel, Switzerland) 20220527 6

<h4>Background</h4>Variants of linker histone H1 are tissue-specific and are responsible for chromatin compaction accompanying cell differentiation, mitotic chromosome condensation, and apoptosis. Heterochromatinization, as the main feature of these processes, is also associated with pronounced trimethylation of histones H3 at the lysine 9 position (H3K9me3).<h4>Methods</h4>By confocal microscopy, we analyzed cell cycle-dependent levels and distribution of phosphorylated histone H1 (H1ph) and H3 ...[more]

PMID: 35743829

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