Proteomics

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SILAC proteomic analysis of secreted proteins from PLC cells expressing EV or PRSS35

ABSTRACT: Since PRSS35 only exhibited tumor suppressive effects in mice, but not in the cultured cells, we speculated that some factors in the microenvironment are affected by PRSS35. To explore this possibility, we performed SILAC proteomic analysis in conjunction with RNA sequencing to identify secreted proteins that decreased in accumulation, but not in transcription level, in the culture medium of PRSS35 overexpressing PLC cells.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Ting Wang  

LAB HEAD: Huafeng Zhang

PROVIDER: PXD033579 | Pride | 2023-02-15

REPOSITORIES: Pride

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Action DRS
Database-uniprot-secreted-filtered-reviewed_yes_AND_organism__Homo_sapiens.xlsx Xlsx
R1_WT_1_PLC_SILAC_1_20191212.raw Raw
R1_WT_1_PLC_SILAC_2_20191212.raw Raw
R1_WT_1_PLC_SILAC_3_20191212.raw Raw
R1_WT_1_PLC_SILAC_4_20191212.raw Raw
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