Top-Down proteomics and Bottom-Up terminomics for the Detection of Protein Termini in C. elegans

ABSTRACT: Here we investigate how top-down proteomics (TDP) can be of value for the detection of protein termini. Either GELFrEE or solid-phase enrichment was conducted for pre-fraction. To verify the detected N-termini HUNTER (High-efficiency Undecanal-based N Termini EnRichment) was employed. Reductive dimethylation on intact protein level was additionally applied.

INSTRUMENT(S):

ORGANISM(S): Caenorhabditis Elegans

SUBMITTER: Andreas Tholey  

LAB HEAD: Andreas Tholey

PROVIDER: PXD033668 | Pride | 2022-09-26

REPOSITORIES: Pride