Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Cx32 surface expression (wildtype vs. mutants)

ABSTRACT: Cx32 wildtype and mutants (W3S, R22G) were expressed in HEK293F cells and subjected to surface biotinylation to check for expression level differences between the different conditions. A CFP control was conducted.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Dina Schuster

LAB HEAD: Volodymyr Korkhov

PROVIDER: PXD033671 | Pride | 2023-08-08

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	20220502_165304_dschuster.sne	Other
	20220502_221805_dschuster_Report.csv	Csv
	20220502_221805_dschuster_Report.log.txt	Txt
	20220502_221805_dschuster_Report.params	Other
	20220502_221805_dschuster_Report.setup.txt	Txt

Items per page:

1 - 5 of 29

Similar Datasets

Cx32 surface expression (wildtype vs. mutants) - HeLa cells

Project description:Cx32 wildtype and mutants (W3S, R22G) were expressed in HeLa cells and subjected to surface biotinylation to check for expression level differences between the different conditions. A CFP control was conducted.

2023-08-08 | PXD039379 | Pride

Cx32 wt vs mutant purified proteins

Project description:An analysis of purified Cx32 wt, W3S and R22G mutations. The three different proteins were expressed, digested and measured in DIA.

2023-08-08 | PXD033848 | Pride

Cx43 purified protein analysis (N-term analysis)

Project description:Purified Cx43 was analyzed to check for modifications of its N-terminus to aid structural studies.

2023-07-27 | PXD033824 | Pride

HOT regions are CpG dense promoters in C. elegans and humans

Project description:ChIP seq of Cfp-1 and H3K4me3 in C. elegans late embryos The coding region of F52B11.1a (cfp-1) was PCR amplified from N2 genomic DNA using Phusion polymerase (Finnzymes) and Gateway cloned into pDONR221. The cfp-1 coding region was then recombined into the MosSCI compatible vector pCFJ201 (which targets Mos site Mos1(cxTi10882) chrIV ) downstream of the dpy-30 promoter and upstream of gfp::tbb-2 3’UTR (Zeiser et al. 2011) to generae strain JA1597 expressing GFP tagged Cfp-1 protein. Late embryos were obtained by aging embryos collected by hypochlorite treatment 3.5 hrs prior to flash freezing in liquid nitrogen. Formaldehyde-fixed chromatin extracts and chromatin immunoprecipitations were as in (Kolasinska-Zwierz et al. 2009) except that DNA was sonicated to a size range of 200-400bp. ChIP assays were performed in 1 ml extract (1 mg protein) in FA buffer with 10 micrograms of anti-GFP rabbit serum (Abcam ab290) and anti-H3K4me3 (Abcam ab8580) individually. DNA sequencing libraries were constructed according using the Illumina Truseq sequencing kit and were sequenced on the Illumina platform.

2014-01-01 | E-GEOD-49870 | biostudies-arrayexpress

Surface biotinylation HEK293 Cx32

Project description:The surface expression of Cx32 and other proteins was assessed after transfection.

2025-05-07 | PXD056242 | Pride

Connexin43 null vs wildtype neonatal mouse HEART

Project description:Cy3-labeled cDNA obtained from four pools of three hearts of neonatal C57BL Cx43 null mice were compared to Cy3-labeled cDNA obtained from four pools of three hearts of neonatal C57BL wildtype mice through Cy5-labeled sample reference prepared at once for the entire experiment from aorta, brain, heart, kidney, liver, lung, ovary/testicles, spleen, and stomach - equal amounts from adult male and female C57BL mice. Keywords = Cx32 null vs wildtype neonatal mouse heart Keywords: parallel sample

2004-11-13 | GSE1961 | GEO

CX43 heterozygous, Cx43 null and Cx32 null vs wildtype neonatal mouse brain

Project description:Cy3-labeled cDNA from brains of neonatal C57BL Cx43 null, Cx43 heterozygous and Cx32 null mice were compared among themselves and to Cy3-labeled cDNA from brains of neonatal C57BL wildtype mice through Cy5-labeled sample reference prepared at once for the entire experiment from aorta, brain, heart, kidney, liver, lung, ovary/testicles, spleen, and stomach - equal amounts from adult male and female C57BL mice.

2004-11-12 | GSE1954 | GEO

Connexin 32-mediated cell-cell communication is essential for hepatic differentiation from human embryonic stem cells

Project description:Gap junction-mediated cell-cell interactions are highly conserved and play essential roles in cell survival, proliferation, differentiation and patterning. We report that Connexin 32 (Cx32)-mediated gap junctional intercellular communication (GJIC) is necessary for human embryonic stem cell-derived hepatocytes (hESC-Heps) during step-wise hepatic lineage restriction and maturation. Vitamin K2, previously shown to promote Cx32 expression in mature hepatocytes, up-regulated Cx32 expression and GJIC activation during hepatic differentiation and maturation, resulting in significant increases of hepatic markers expression and hepatocyte functions. In contrast, negative Cx32 regulator 2-aminoethoxydiphenyl borate blocked hESC-to-hepatocyte maturation and muted hepatocyte functions through disruption of GJIC activities. Dynamic gap junction organization and internalization are phosphorylation-dependent and the p38 mitogen-activated protein kinases pathway (MAPK) can negatively regulate Cxs through phosphorylation-dependent degradation of Cxs. We found that p38 MAPK inhibitor SB203580 improved maturation of hESC-Heps correlating with up-regulation of Cx32; by contrast, the p38 MAPK activator, anisomycin, blocked hESC-Heps maturation correlating with down-regulation of Cx32. These results suggested that Cx32 is essential for cell-cell interactions that facilitate driving hESCs through hepatic-lineage maturation. Regulators of both Cx32 and other members of its pathways maybe used as a promising approach on regulating hepatic lineage restriction of pluripotent stem cells and optimizing their functional maturation.

2017-02-12 | GSE76098 | GEO

Connexin43 null vs wildtype neonatal mouse HEART

2010-06-05 | E-GEOD-1961 | biostudies-arrayexpress

CX43 heterozygous, Cx43 null and Cx32 null vs wildtype neonatal mouse brain

2010-06-05 | E-GEOD-1954 | biostudies-arrayexpress