Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Eclipse
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Hepatocyte
SUBMITTER: Qing Yu
LAB HEAD: Steven Gygi
PROVIDER: PXD033679 | Pride | 2023-03-11
REPOSITORIES: Pride
Yu Qing Q Liu Xinyue X Keller Mark P MP Navarrete-Perea Jose J Zhang Tian T Fu Sipei S Vaites Laura P LP Shuken Steven R SR Schmid Ernst E Keele Gregory R GR Li Jiaming J Huttlin Edward L EL Rashan Edrees H EH Simcox Judith J Churchill Gary A GA Schweppe Devin K DK Attie Alan D AD Paulo Joao A JA Gygi Steven P SP
Nature communications 20230202 1
Targeted proteomics enables hypothesis-driven research by measuring the cellular expression of protein cohorts related by function, disease, or class after perturbation. Here, we present a pathway-centric approach and an assay builder resource for targeting entire pathways of up to 200 proteins selected from >10,000 expressed proteins to directly measure their abundances, exploiting sample multiplexing to increase throughput by 16-fold. The strategy, termed GoDig, requires only a single-shot LC- ...[more]