Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparative proteome of a marine dinoflagellate under different temperatures

ABSTRACT: Batch cultures of P. donghaiense were maintained in 0.22 filtered and autoclaved seawater (30 psu) with K medium, and grown under cool white fluorescent light at an irradiance of 100 μmol quanta m-2 s-1 with a 14:10 h light: dark cycle at 25 ℃. Tripicated experiments at 19, 22, 25 and 28 ℃ were performed in 2 L acid-washed and autoclaved polycarbonate bottles containing 1.6 L medium. To obtain stable metabolic activity of cells, P. donghaiense at each temperature were cultured and semi-continuously diluted daily for approximately 20 days before samplings.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Prorocentrum Donghaiense

TISSUE(S): Photosynthetic Cell, Whole Body

SUBMITTER: Hao Zhang

LAB HEAD: Hao Zhang

PROVIDER: PXD033681 | Pride | 2022-08-15

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Similar Datasets

Ctenopharyngodon idella iTRAQ - Understanding the Immune Mechanisms of Vibrio mimicus targeted epitopes gene vaccine by iTRAQ Technology

Project description:Understanding the Immune Mechanisms of Vibrio mimicus targeted epitopes gene vaccine by iTRAQ Technology

2018-12-18 | PXD012011 | Pride

Human HepG2 ITRAQ LC-MS/MS - Integrated proteomic and transcriptomic analysis revealslong noncoding RNA HOTAIR promotes hepatocellular carcinoma cell proliferation by regulating opioid growth factor receptor (OGFr)

Project description:Long non-coding RNA HOX transcript antisense RNA (HOTAIR) is involved in human tumorigenesis and dysregulated in hepatocellular carcinoma (HCC). However, the molecular mechanisms underlying HOTAIR functions in HCC are not well understood. Here, an integrated RNA sequencing transcriptomic and quantitative proteomic analysis were employed to systematically explore the regulatory role of HOTAIR in HCC. A total of 673 transcripts and 298 proteins were identified to be dysregulated after HOTAIR inhibition. Bioinformatics studies indicated that the differentially expressed genes (DEGs) and proteins (DEPs) are involved in many biological processes, especially cancer-related signalling pathways.

2017-11-24 | PXD006554 | Pride

Skeletonema marinoi LC-MS/MS analysis

Project description:Rising atmospheric CO2 concentrations are leading to ocean acidification, altering the inorganic carbon buffer system with consequences for marine organisms. Here we applied RNA-seq and iTRAQ quantification to investigate the potential impacts of ocean acidification on the temperate coastal marine diatom Skeletonema marinoi.

2020-12-09 | PXD021416 | Pride

Generation of zero-valent sulfur from dissimilatory sulfate reduction in sulfate-reducing microorganisms

Project description:Dissimilatory sulfate reduction (DSR) mediated by sulfate-reducing microorganisms (SRMs) plays a pivotal role in global sulfur, carbon, oxygen, and iron cycles since ~3.5 billion years ago. The canonical DSR pathway is believed to be sulfate reduction to sulfide. Herein, we report a new DSR pathway in phylogenetically diverse SRMs through which zero-valent sulfur (ZVS) is directly generated. We identified that approximately 8.9% of sulfate reduction was directed toward ZVS with S8 as a predominant product, and the ratio of sulfate-to-ZVS could be changed with SRMs’ growth conditions, particularly the medium salinity. Further coculturing experiments and metadata analyses revealed that DSR-derived ZVS supported the growth of various ZVS-metabolizing microorganisms, highlighting this new pathway as an essential component of the sulfur biogeochemical cycle

2023-04-28 | PXD040825 | Pride

An Isocaloric High-Fat Diet Improves Lifespan in Rats and Drosophila

Project description:The health effect of dietary fat has been one of the most vexing issues in the nutrition field. Few animal studies have examined the impact of high-fat diets on lifespan by controlling energy intake. In this study, we found that compared to a normal diet, an isocaloric high-fat diet (IHF) significantly prolonged lifespan by decreasing the profiles of free fatty acids (FFAs) in serum and multiple tissues by downregulating FFAs anabolism and upregulating catabolism pathways in rats and flies. Proteomics analysis in rats identified PPRC1 as a key protein that was significantly upregulated by nearly 2-fold by IHF, and among the FFAs, only palmitic acid (PA) was robustly and negatively associated with the expression of PPRC1. Using PPRC1 transgenic RNAi/overexpression flies and in vitro experiments, we further demonstrated that IHF significantly reduced PA, which could upregulate PPRC1 through PPARG, resulting in improvements in oxidative stress and inflammation and prolonging lifespan.

2021-01-06 | PXD022585 | Pride

Scophthalmus maximus spleen iTRAQ

Project description:Scophthalmus maximus spleen proteome after polyI:C-injection under high or low temperature were compared by iTRAQ(isobaric tags for relative and absolute quantitation).

2018-02-07 | PXD005293 | Pride

Liver proteome response of turbot to lysine and leucine in free and dipeptide forms

Project description:In this iTRAQ quantification project, there were 6 Scophthalmus_maximus samples named CAA-1, CAA-2, CAA-3, Lys-Leu-1, Lys-Leu-2, Lys-Leu-3 and we did one technical duplicate experiments. Totally 935928 spectrums were generated, 32261 peptides and 5708 proteins were identified with 1% false discovery rate (FDR).

2021-06-22 | PXD025276 | Pride

iTRAQ-based Quantitative Proteomics Analysis Screen for Critical Proteins during Temperature-Responsive Flowering of Crocus sativus

Project description:To uncover the underlying mechanism of temperature-responsive flowering in Crocus sativus and unveil flowering regulatory proteins, iTRAQ (isobaric tags for relative or absolute quantitation)-based proteomics were applied to study the differences in protein expression profiles in flowering and non-flowering saffron grown at room- and low-temperatures, respectively. A total of 5,624 proteins were identified，and 201 proteins showed differential accumulations between the flowering and non-flowering groups.

2021-09-09 | PXD021020 | Pride

Synechococcus IBT LC-MS/MS - Proteomic Response to Rising Temperature in the Marine Cyanobacterium Synechococcus Grown in Different Nitrogen Sources

Project description:we investigated molecular response of an oceanic Synechococcus strain WH 8102 grown on two nitrogen sources (nitrate and urea) under present (25°C) and predicted future (28°C) temperature conditions using an IBT-based quantitative proteomic approach.

2019-08-19 | PXD011884 | Pride

Extracellular vesicles secreted from senescent cancer cells promote colorectal cancer progression through SERPINE1

Project description:By the iTRAQ-based quantitative proteomics, we assessed the protein cargos inside EVs secreted from colorectal cancer cells

2024-04-06 | PXD047062 | Pride