Proteomics

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Quantitative proteomic study on whole protein TMT labeling of wheat samples


ABSTRACT: Protein extraction, Trypsin Hydrolysis, TMT labeling, LC-MS analysis, database search, mass spectrometry quality control detection and bioinformatics analysis were performed in each treatment group.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Triticum Aestivum (wheat)

TISSUE(S): Root, Shoot

SUBMITTER: Yuqi Li  

LAB HEAD: Yuqi Li

PROVIDER: PXD033767 | Pride | 2022-10-15

REPOSITORIES: Pride

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Publications

Physiological and Differential Proteomic Analysis at Seedling Stage by Induction of Heavy-Ion Beam Radiation in Wheat Seeds.

Li Yuqi Y   Gu Jiayu J   Irshad Ahsan A   Zhao Linshu L   Guo Huijun H   Xiong Hongchun H   Xie Yongdun Y   Zhao Shirong S   Ding Yuping Y   Zhou Libin L   Kong Fuquan F   Fang Zhengwu Z   Liu Luxiang L  

Frontiers in genetics 20220719


Novel genetic variations can be obtained by inducing mutations in the plant which help to achieve novel traits. The useful mutant can be obtained through radiation mutation in a short period which can be used as a new material to produce new varieties with high yield and good quality wheat. In this paper, the proteomic analysis of wheat treated with different doses of <sup>12</sup>C and <sup>7</sup>Li ion beam radiation at the seedling stage was carried out through a Tandem Mass Tag (TMT) taggin  ...[more]

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