Dataset Information

Liquid chromatography-tandem mass spectrometry analysis of bone marrow from the ground or running rats

ABSTRACT: Exercise can prevent osteoporosis and improve immune function, but the mechanism remains unclear. To explore the key factors involved in these two processes, we collected bone marrow from ground and running rats and then conducted liquid chromatography-tandem mass spectrometry.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Bone Marrow

SUBMITTER: Hui Peng

LAB HEAD: Xianghang Luo

PROVIDER: PXD033807 | Pride | 2023-10-17

REPOSITORIES: Pride

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Publications

A mechanosensitive lipolytic factor in the bone marrow promotes osteogenesis and lymphopoiesis.

Peng Hui H Hu Biao B Xie Ling-Qi LQ Su Tian T Li Chang-Jun CJ Liu Ya Y Yang Mi M Xiao Ye Y Feng Xu X Zhou Rui R Guo Qi Q Zhou Hai-Yan HY Huang Yan Y Jiang Tie-Jian TJ Luo Xiang-Hang XH

Cell metabolism 20220614 8

Exercise can prevent osteoporosis and improve immune function, but the mechanism remains unclear. Here, we show that exercise promotes reticulocalbin-2 secretion from the bone marrow macrophages to initiate bone marrow fat lipolysis. Given the crucial role of lipolysis in exercise-stimulated osteogenesis and lymphopoiesis, these findings suggest that reticulocalbin-2 is a pivotal regulator of a local adipose-osteogenic/immune axis. Mechanistically, reticulocalbin-2 binds to a functional receptor ...[more]

PMID: 35705079

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Project description:The aim of this experiment was to explore transcriptomic changes in the gills of Altantic salmon (Salmo salar) following a model lab based parr smolt transformation from fresh water (FW) to salt water (SW). The process of smoltification (migration from FW to SW) is stimulated by long day photoperiod, which acts on the tissue-specific levels of active thyroid hormone (triiodothyronine, T3) through the expression of thyroid hormone type 2 deiodinase (dio2), responsible for conversion of inactive thyroxine (T4) to T3. To gain insight into the functional significance of dio2 induction, we performed a SW-challenge experiment in which we inhibited dio2 activity by addition of iopanoic acid (IOP) to the SW. We also assessed the ability of co-treatment with T3 to override IOP effects. Juvenile fish maintained in FW were subjected to a standard smolt photoperiod regime known to stimulate smoltification, after which they were randomly allocated to one of the four treatments: SW, SW+IOP, SW+IOP+T3 and FW as a control. Fish (n = 8-10 per treatment) were exposed to these conditions for 6 h and then sacrificed to obtain gill tissue for microarray analysis, carried out using a custom-designed Agilent oligonucleotide microarray platform Salar_2 (one glass slide with 4 x 44K arrays, Agilent design ID: 025520, array design A-MEXP-2065), developed and validated for Atlantic salmon (for details, see specific protocols) . In total, 36 hybridisations were performed on gills from individual fish, with 8-10 replicate fish per treatment. We identified 1939 genes whose expression was significantly increased or decreased by transfer from FW to SW. For a subset of 259 genes, this SW response was abolished if IOP was added to the SW, but maintained if T3 was also present during IOP treatment. This group of genes constitutes a candidate list, for which SW-inducibility appears to be dependent on locally mediated changes in gill T3 availability. The results of this experiment have been submitted for publication in Current Biology and are currently under review.

Dataset Information

Liquid chromatography-tandem mass spectrometry analysis of bone marrow from the ground or running rats

Publications

A mechanosensitive lipolytic factor in the bone marrow promotes osteogenesis and lymphopoiesis.

Similar Datasets

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