Dataset Information

Murine and human bile proteome analysis

ABSTRACT: The gut microbiota is known to influence host metabolism, but its impact on lipid uptake from the nutrition and underlying mechanisms remain mysterious. Combining lipidomics and proteomics we discovered a microbiome-related phospholipase A1 (PLA1) activity in bile.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Bile

SUBMITTER: Piero Giansanti

LAB HEAD: Bernhard Kuster

PROVIDER: PXD033894 | Pride | 2026-07-30

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	4444_GD1_P051198_S00_10uL_R1.raw	Raw
	4444_GD1_P051198_S00_15uL_R1.raw	Raw
	4444_GD1_P051198_S00_1uL_R1.raw	Raw
	4444_GD1_P051198_S00_4uL_R1.raw	Raw
	4444_GD1_P051199_S00_10ul_R1.raw	Raw

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Publications

Gut microbiota restricts intestinal lipid uptake via modulation of bile phosphatidylcholine metabolism in mice.

Brunner Sarah S Plagge Johannes J Zimmermann-Kogadeeva Maria M Höring Marcus M Liebisch Gerhard G Basic Marijana M Bolsega Silvia S Janssen Klaus-Peter KP Slack Emma E von Gamm Sophia S Viehof-Beckmann Alina A Clavel Thomas T Zimmermann Michael M Heeren Joerg J Giansanti Piero P Weiss Anna S AS Hermeling Sven S Dupont Aline A Ullrich Anna-Lena AL Jokisch Florian F Seeliger Claudine C Bleich Andre A Hidrobo Maria M Stecher Bärbel B Coleman Olivia I OI Moresi Claudia C Greter Giorgia G Arnoldini Markus M Scheiber Josef J Matysik Silke S Klingenspor Martin M Küster Bernhard B Haller Dirk D Burkhardt Ralph R Kuipers Folkert F Ecker Josef J

Nature microbiology 20260729 8

The gut microbiota influences host metabolism, but the mechanisms of lipid uptake from food remain mysterious. Here we used stable isotope-labelled tracers in gnotobiotic mouse models, which revealed that host uptake of dietary lipids depends on microbial colonization. Systemic lipid metabolism modelling predicted that the gut microbiota restricts intestinal lipid absorption, and labelled lipid administration verified that the gut contents of microbiota-colonized mice contained up to 12-fold mor ...[more]

PMID: 42527633

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