Identification of dGTP-interacting mitochondrial proteins by affinity purification and LC-ESI-MS/MS
Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Velos
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Liver
SUBMITTER: 
Yolanda Cámara
LAB HEAD: Ramon Martí
PROVIDER: PXD033900 | Pride | 2022-06-29
REPOSITORIES: Pride
Publications
Most mitochondrial dGTP is tightly bound to respiratory complex I through the NDUFA10 subunit.
Molina-Granada David D González-Vioque Emiliano E Dibley Marris G MG Cabrera-Pérez Raquel R Vallbona-Garcia Antoni A Torres-Torronteras Javier J Sazanov Leonid A LA Ryan Michael T MT Cámara Yolanda Y Martí Ramon R
Communications biology 20220623 1
Imbalanced mitochondrial dNTP pools are known players in the pathogenesis of multiple human diseases. Here we show that, even under physiological conditions, dGTP is largely overrepresented among other dNTPs in mitochondria of mouse tissues and human cultured cells. In addition, a vast majority of mitochondrial dGTP is tightly bound to NDUFA10, an accessory subunit of complex I of the mitochondrial respiratory chain. NDUFA10 shares a deoxyribonucleoside kinase (dNK) domain with deoxyribonucleosi ...[more]
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