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Proteomic characterization and protective effect against brain ischemia produced by adult mouse neural progenitor cells-derived extracellular vesicles.

ABSTRACT: Hypoxia produced by stroke activates the proliferation of neural progenitor cells (NPC) in the subventricular zone of the adult mouse brain. Newly generated cells seem to participate in the protection of the ischemic brain through the release of extracellular vesicles (EVs) that might target different cell types and modify their function by delivering proteins, lipids, and nucleic acids. We investigated the possible communication route mediated by EVs between NPC and neurons under conditions of cerebral ischemia using an in vitro model of oxygen and glucose deprivation (OGD). We collected OGD-activated NPC-derived EVs by differential centrifugation of the conditioned media in which NPC were grown for 12 h after OGD, and as a control, we harvested EVs released by cells cultured under normoxic conditions. EVs were characterized by shape and size with transmission electron microscopy and NanoTracking assays, and the presence of typical exosome markers was assessed by western blot. The proteomic profiling of NPC-derived EVs was carried out after resolving the proteins by polyacrylamide gel electrophoresis and performed in-gel digestion with trypsin, then the identity of the products was determined by tandem mass spectrometry (ESI-IMS-MS). Overrepresentation analyses of the proteomic data allowed us to identify executioners of signaling pathways involved in neuroprotection. (Supported by DGAPA-PAPIIT IN207020 and CONACYT A1-S-13219).

INSTRUMENT(S): SYNAPT G2-Si

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Neural Progenitor Cell

DISEASE(S): Stroke

SUBMITTER: Emmanuel Rios-Castro

LAB HEAD: Luis B. Tovar y Romo

PROVIDER: PXD033915 | Pride | 2023-07-14

REPOSITORIES: Pride

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Proteomic characterization and protective effect against brain ischemia produced by adult mouse neural progenitor cells-derived extracellular vesicles.

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