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Life-time study of premodified histone for Identification of chromatinized proteome defined by site-specific histone acylations in living cells

ABSTRACT: C13, N15 isotope labelled heavy lysine bearing the modification group, such as cr and bhb, were site-sepcifically incorporated into histone H3K56 in living cells for measurement of the modification life of H3K56cr and H3K56bhb without iinterference caused by endogenous histone modification. Utilizaing mass spectrometry, we compared with H3K56cr/bhb peptide with the H3K79 peptide as internal standard at different times. The quantitation analysis of H3K56cr and H3K56bhb at different time points can be comparative.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Early Embryonic Cell

SUBMITTER: Fangfei Qin

LAB HEAD: Peng R. Chen

PROVIDER: PXD033958 | Pride | 2023-03-06

REPOSITORIES: Pride

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Linking chromatin acylation mark-defined proteome and genome in living cells.

Qin Fangfei F Li Boyuan B Wang Hui H Ma Sihui S Li Jiaofeng J Liu Shanglin S Kong Linghao L Zheng Huangtao H Zhu Rongfeng R Han Yu Y Yang Mingdong M Li Kai K Ji Xiong X Chen Peng R PR

Cell 20230301 5

A generalizable strategy with programmable site specificity for in situ profiling of histone modifications on unperturbed chromatin remains highly desirable but challenging. We herein developed a single-site-resolved multi-omics (SiTomics) strategy for systematic mapping of dynamic modifications and subsequent profiling of chromatinized proteome and genome defined by specific chromatin acylations in living cells. By leveraging the genetic code expansion strategy, our SiTomics toolkit revealed di ...[more]

PMID: 36868209

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