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Identification of lysine-lactylated substrates in gastric cancer cells

ABSTRACT: Lysine lactylation is a newly discovered post-translational modification (PTM) in histones and plays important roles in regulating gene expression. However, Kla in non-histone proteins in mammal is still unclear. Here, an analysis of lactylated proteins in gastric cancer AGS cells by using LC-MS/MS was conducted, and 2375 Kla sites on 1014 proteins were identified.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Xianliang Ma

LAB HEAD: Yongbin Ding

PROVIDER: PXD033991 | Pride | 2022-08-11

REPOSITORIES: Pride

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Identification of lysine-lactylated substrates in gastric cancer cells.

Yang Dawei D Yin Jie J Shan Liuqun L Yi Xingling X Zhang Wei W Ding Yongbin Y

iScience 20220617 7

Cancer cells tend to utilize aerobic glycolysis to generate energy and metabolites; the end product of aerobic glycolysis is lactate, which promotes lysine lactylation (Kla). Kla is a newly discovered histone post-translational modification (PTM) that plays important roles in regulating gene expression. However, Kla in non-histone mammalian proteins is unclear. Here, a comprehensive analysis of lactylated proteins in gastric cancer AGS cells was conducted. There were 2375 Kla sites found in 1014 ...[more]

PMID: 35800753

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