Mass spectrometry analysis of unconventional kinetoplastid kinetochore proteins KKT1, KKT2, and KKT6

ABSTRACT: The kinetochore is the macromolecular protein machine that drives chromosome segregation in eukaryotes. Here we performed chemical crosslinking mass spectrometry of the Trypanosoma brucei KKT2 DPB/KKT1C complex and KKT1/KKT6 complex. We also performed immunoprecipitation and mass spectrometry of KKT1 fragments (KKT1N and KKT1C) that were ectopically expressed and purified from trypanosomes.

INSTRUMENT(S):

ORGANISM(S): Trypanosoma Brucei

SUBMITTER: Bungo Akiyoshi  

LAB HEAD: Bungo Akiyoshi

PROVIDER: PXD034039 | Pride | 2022-06-10

REPOSITORIES: Pride