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The eWAT proteomics of wild type mice and Mdm2 adipocyte-specific knock-in mice fed high-fat diet

ABSTRACT: The epididymal adipose tissue (eWAT) samples from wild type mice fed high-fat diet for 12 weeks (H_WT_E) and Mdm2 adipocyte-specific knock-in mice fed high-fat diet for 12 weeks (H_KI_E) were mixed separately, and subjected to proteomic study by Label-free quantitative techniques and mass spectrometry-based proteomics techniques, etc. The proteomics of mixed eWAT samples were performed in Jingjie PTM BioLab (Hangzhou) Co. Ltd (www.ptm-biolab.com.cn). The difference was determined by 1.5-fold-change criterion, FDR < 0.01.

INSTRUMENT(S): LTQ Orbitrap, Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Whole Body

SUBMITTER: Xiaojun Liu

LAB HEAD: Xiaojun Liu

PROVIDER: PXD034074 | Pride | 2022-08-11

REPOSITORIES: Pride

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Murine double minute 2 aggravates adipose tissue dysfunction through ubiquitin-mediated six-transmembrane epithelial antigen of prostate 4 degradation.

Zhao Wei W Xu Qiang Q Yang Jiahui J Xie Xianghong X Li Chunmei C Zhang Weihong W Chen Enhui E Guo Yanfang Y Gao Mingyue M Shi Jie J Zhang Huabing H Yao Hong H Li Meixia M Yan Li L Fang Fude F Wu Wenming W Liu Xiaojun X

iScience 20220607 7

Healthy adipose tissue is crucial to maintain normal energy homeostasis. Little is known about the role of murine double minute 2 (MDM2), an E3 ubiquitin ligase and has been highlighted in oncopathology, in adipose tissue. Our results indicated that MDM2 expression was associated with nutritional status. <i>Mdm2</i> adipocyte-specific knock-in (<i>Mdm2</i>-AKI) mice exhibited exacerbated weight gain, insulin resistance, and decreased energy expenditure. Meanwhile, chronic high-fat diet (HFD) exp ...[more]

PMID: 35747386

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