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Lysine acetylation contributes to artificial ageing in wheat (Triticum aestivum L.) seeds.

ABSTRACT: In this work, the role of protein acetylation modification in wheat aging was studied using TMT labelling, enrichment of acetylation peptides and quantitative proteomics strategy of high-resolution liquid chromatography-mass spectrometry. A total of 1663 acetylation sites were identified on 892 proteins, of which 1526 sites on 830 proteins had quantitative information. Furthermore, 255 differentially acetylated sites were identified, of which 135 sites were up-regulated and 120 sites were down-regulated.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Triticum Aestivum (wheat)

SUBMITTER: Xianliang Ma

LAB HEAD: Yangyong Lv

PROVIDER: PXD034134 | Pride | 2023-03-10

REPOSITORIES: Pride

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Quantitative Acetylome Analysis of Soft Wheat Seeds during Artificial Ageing.

Liang Liuke L Xie Aowen A Yang Haojie H Li Na N Ma Ping'an P Wei Shan S Zhang Shuaibing S Lv Yangyong Y Hu Yuansen Y

Foods (Basel, Switzerland) 20221112 22

Lysine acetylation (Kac) is a protein post-translational modification (PTM) widely found in plants that plays vital roles in metabolic pathways. Although seed germination and development are regulated by Kac, its potential function in seed ageing remains to be investigated. Our preliminary study demonstrated that Kac levels were altered during wheat seed artificial ageing. However, its specific role in this process still needs to be elucidated. Here, we performed quantitative acetylation proteom ...[more]

PMID: 36429203

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