Proteomics

Dataset Information

0

RNA pulldown-MS, Dlg4 RNA in mHippoE-2 cells


ABSTRACT: Goal was to identify RNA-binding proteins with a preference for m6A-modified RNA using an RNA bait generated from a known m6A site in the Dlg4 (PSD-95) mRNA 3`-UTR. By comparison of differential enrichment between m6A-RNA pulldown fractions and A-RNA or no RNA fractions, we can identify m6A-binding proteins.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Dendritic Cell

SUBMITTER: Kate Meyer  

LAB HEAD: Kate Meyer

PROVIDER: PXD034161 | Pride | 2022-10-13

REPOSITORIES: Pride

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Publications

RBM45 is an m<sup>6</sup>A-binding protein that affects neuronal differentiation and the splicing of a subset of mRNAs.

Choi Seung H SH   Flamand Mathieu N MN   Liu Bei B   Zhu Huanyu H   Hu Meghan M   Wang Melanie M   Sewell Jonathon J   Holley Christopher L CL   Al-Hashimi Hashim M HM   Meyer Kate D KD  

Cell reports 20220801 9


N<sup>6</sup>-methyladenosine (m<sup>6</sup>A) is deposited co-transcriptionally on thousands of cellular mRNAs and plays important roles in mRNA processing and cellular function. m<sup>6</sup>A is particularly abundant within the brain and is critical for neurodevelopment. However, the mechanisms through which m<sup>6</sup>A contributes to brain development are incompletely understood. RBM45 acts as an m<sup>6</sup>A-binding protein that is highly expressed during neurodevelopment. We find that  ...[more]

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