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Global proteome comparison between MEF cells and mESC-R1 cells

ABSTRACT: The aim of this project is to use an integrated approach involving label-free strategy, HPLC fractionation and LC-MS/MS to quantify the dynamic changes of the whole proteome of Mouse Cell(MEF and R1 cells).

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Fangyuan Cheng

LAB HEAD: Mian Wu

PROVIDER: PXD034181 | Pride | 2023-03-10

REPOSITORIES: Pride

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P21-Activated Kinase 4 Pak4 Maintains Embryonic Stem Cell Pluripotency via Akt Activation.

Cheng Fangyuan F Li Mingyue M Thorne Rick Francis RF Liu Guangzhi G Zhang Yuwei Y Wu Mian M Liu Lianxin L

Stem cells (Dayton, Ohio) 20221001 10

Exploiting the pluripotent properties of embryonic stem cells (ESCs) holds great promise for regenerative medicine. Nevertheless, directing ESC differentiation into specialized cell lineages requires intricate control governed by both intrinsic and extrinsic factors along with the actions of specific signaling networks. Here, we reveal the involvement of the p21-activated kinase 4 (Pak4), a serine/threonine kinase, in sustaining murine ESC (mESC) pluripotency. Pak4 is highly expressed in R1 ESC ...[more]

PMID: 35896382

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