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Passive elution of proteoforms from polyacrylamide gels combined with anion exchange purification for in-depth top-down analysis of the human low molecular weight proteome.

ABSTRACT: We developed a novel sample preparation method by combining Passively Eluting Proteins from Polyacrylamide gels as Intact species for MS (PEPPI-MS) fractionation with Anion-Exchange disc-assisted Sequential sample Preparation (AnExSP) purification, and evaluated its performance in TDP analysis by comparing it with a conventional PEPPI workflow using Methanol-Chloroform-Water (MCW) precipitation.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Andreas Tholey

LAB HEAD: Andreas Tholey

PROVIDER: PXD034353 | Pride | 2022-09-26

REPOSITORIES: Pride

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