Dataset Information

Proteomic analysis of two citrus rootstocks subjected to multiple abiotic stress conditions

ABSTRACT: Drought, heat and high light irradiation are abiotic stresses that negatively affect plant development and reduce crop productivity. The confluence of these three factors is common in nature, causing extreme situations for plants that compromise their viability. Drought and heat stress increase the saturation of the photosystem’s reaction centers, increasing sensitivity to high light irradiation. In addition, these stress conditions affect PSII integrity, alter redox balance of the electron transport chain and decrease photosynthetic rate. Here, we studied the effect of drought, high light and heat stress factorial stress combination on the photosynthetic apparatus of two citrus rootstocks, Carrizo citrange (Citrus sinensis × Poncirus trifoliata) and Cleopatra mandarin (Citrus reshni). Proteomic data, together with transcriptomic, showed the main responses of citrus plant to multiple abitoic stress conditions. The enhancement of KEGG pathways such as RNA metabolic processes and the accumulation of family proteins like HSPs were key responses to face the combination of these abiotic stress factors.

INSTRUMENT(S): SYNAPT G2-Si

ORGANISM(S): Citrus Reshni Citrus Sinensis X Citrus Trifoliata

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Aurelio Gómez-Cadenas

LAB HEAD: Aurelio Gómez Cadenas

PROVIDER: PXD034419 | Pride | 2023-03-11

REPOSITORIES: Pride

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Publications

Reduction of heat stress pressure and activation of photosystem II repairing system are crucial for citrus tolerance to multiple abiotic stress combination.

Balfagón Damián D Zandalinas Sara I SI Dos Reis de Oliveira Tadeu T Santa-Catarina Claudete C Gómez-Cadenas Aurelio A

Physiologia plantarum 20221101 6

Drought, heat and high irradiance are abiotic stresses that negatively affect plant development and reduce crop productivity. The confluence of these three factors is common in nature, causing extreme situations for plants that compromise their viability. Drought and heat stresses increase the saturation of the photosystem reaction centers, increasing sensitivity to high irradiance. In addition, these stress conditions affect photosystem II (PSII) integrity, alter redox balance of the electron t ...[more]

PMID: 36309819

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Project description:Purpose: Brassica. juncea is vulnerable to abiotic stresses at specific stages in its life cycle. However, till date no attempts have been made to elucidate the genome-wide changes in the transcriptome of B. juncea subjected to either high temperature or drought stress. Hence, to gain global insights into genes, transcription factors and kinases regulated by these stresses and to provide basic information on coding transcripts that are associated with traits of agronomic importance, we utilized a combinatorial approach of next generation sequencing and de novo assembly to discover B. juncea transcriptome associated with high temperature and drought. Results: We constructed and sequenced three transcriptome libraries namely Brassica control (BC), Brassica high temperature stress (BHS) and Brassica drought stress (BDS) from control, high temperature treated and drought treated seedlings of Brassica juncea. More than 180 million purity filtered reads were generated which were processed through quality parameters and high quality reads were assembled de-novo using SOAPde-novo assembler. A total of 77750 unique transcripts were identified out of which 69,245 (89%) were annotated with high confidence. We established a subset of 19110 transcripts, which were differentially regulated by either high temperature and/or drought stress. Furthermore, 886 and 2834 transcripts that code for transcription factors and kinases, respectively, were also identified. Investigation of identified transcription factors revealed that 92 responded to high temperature, 72 exhibited alterations in expression during drought stress, and 60 were commonly associated with both the stresses. Similarly, 217, 259 and 193 kinases were responsive to high temperature, drought or both stresses, respectively. Maximum number of up-regulated transcription factors in high temperature and drought stress belonged to heat shock factors (HSFs) and dehydration responsive element-binding (DREB) families respectively. We also identified 239 metabolic pathways, which were perturbed during high temperature and drought treatments. Analysis of gene ontologies associated with differentially regulated genes forecasted their involvement in diverse biological processes. Conclusions: Our study provides first comprehensive discovery of B. juncea transcriptome under high temperature and drought stress conditions. Transcriptome resources generated in this study will enhance our understanding on the molecular mechanisms involved in defining the response of B. juncea against two important abiotic stresses. Furthermore this information would benefit designing of efficient crop improvement strategies for tolerance against conditions of high temperature regimes and water scarcity. Total three RNA-Seq libraries were prepared and sequenced independently [B. juncea control (BC), B. juncea high temperature stressed (BHS) and B. juncea drought stressed (BDS) on Illumina GAIIx sequencer].

Dataset Information

Proteomic analysis of two citrus rootstocks subjected to multiple abiotic stress conditions

Publications

Reduction of heat stress pressure and activation of photosystem II repairing system are crucial for citrus tolerance to multiple abiotic stress combination.

Similar Datasets

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