Identification of cell membrane proteins involved in dsRNA internalization

ABSTRACT: Cellular uptake of dsRNA from the environment is a widespread phenomenon among insects. In Drosophila and in honeybees, the injection and the ingestion respectively of dsRNA against a viral sequence is sufficient to hinder virus replication. We wanted to identify cell surface proteins that bind to extracellular dsRNA, mediating its internalization. To this end, we developed an unconventional co-immunoprecipitation protocol followed by proteomics analysis.

INSTRUMENT(S):

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Cell Culture, Embryonic Stem Cell

SUBMITTER: Yann Verdier  

LAB HEAD: Joelle Vinh

PROVIDER: PXD034508 | Pride | 2025-04-11

REPOSITORIES: Pride