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Integrin beta4 positive versus integrin beta4 negative human keratinocytes

ABSTRACT: Proteomics analysis of sorted human basal and suprabasal keratinocytes to confirm the distinct protein expression in the sorted cell populations.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Keratinocyte, Skin

SUBMITTER: Luca Pontiggia

LAB HEAD: Thomas Biedermann

PROVIDER: PXD034543 | Pride | 2022-08-12

REPOSITORIES: Pride

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Human Basal and Suprabasal Keratinocytes Are Both Able to Generate and Maintain Dermo-Epidermal Skin Substitutes in Long-Term In Vivo Experiments.

Pontiggia Luca L Ahuja Akshay Kumar AK Yosef Hesham Kamaleldin HK Rütsche Dominic D Reichmann Ernst E Moehrlen Ueli U Biedermann Thomas T

Cells 20220709 14

The basal layer of human interfollicular epidermis has been described to harbour both quiescent keratinocyte stem cells and a transit amplifying cell population that maintains the suprabasal epidermal layers. We performed immunofluorescence analyses and revealed that the main proliferative keratinocyte pool in vivo resides suprabasally. We isolated from the human epidermis two distinct cell populations, the basal and the suprabasal keratinocytes, according to the expression of integrin β4 (iβ4). ...[more]

PMID: 35883599

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