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O-linked and N-linked analysis of PTP69D (Drosophila melanogaster)

ABSTRACT: Identifying N-linked and O-linked sites of glycosylation on PTP69D (Drosophila melanogaster) by sHCD and CID neutral loss-trigged MS3.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

SUBMITTER: Robert Bridger

LAB HEAD: Robert Bridger

PROVIDER: PXD034563 | Pride | 2023-03-11

REPOSITORIES: Pride

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Protein tyrosine phosphatase 69D is a substrate of protein O-mannosyltransferases 1-2 that is required for the wiring of sensory axons in Drosophila.

Monagas-Valentin Pedro P Bridger Robert R Chandel Ishita I Koff Melissa M Novikov Boris B Schroeder Patrick P Wells Lance L Panin Vladislav V

The Journal of biological chemistry 20230110 3

Mutations in protein O-mannosyltransferases (POMTs) result in severe brain defects and congenital muscular dystrophies characterized by abnormal glycosylation of α-dystroglycan (α-Dg). However, neurological phenotypes of POMT mutants are not well understood, and the functional substrates of POMTs other than α-Dg remain unknown. Using a Drosophila model, here we reveal that Dg alone cannot account for the phenotypes of POMT mutants, and identify Protein tyrosine phosphatase 69D (PTP69D) as a gene ...[more]

PMID: 36634851

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O-linked and N-linked analysis of PTP69D (Drosophila melanogaster)

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