Proteomics

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Metaproteomic profiling of induced and spontaneous sputum samples from cystic fibrosis patients


ABSTRACT: Application of a mass spectrometry -based approach to assess the lung microbiota composition and the associated metaproteomic functionality of subjects with cystic fibrosis

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Archaea Bacteria Viruses Eukaryota (eucaryotes)

SUBMITTER: Jean ARMENGAUD  

LAB HEAD: Jean Armengaud

PROVIDER: PXD034628 | Pride | 2023-02-04

REPOSITORIES: Pride

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Publications

Quick and wide-range taxonomical repertoire establishment of the cystic fibrosis lung microbiota by tandem mass spectrometry on sputum samples.

Hardouin Pauline P   Pible Olivier O   Marchandin Hélène H   Culotta Karen K   Armengaud Jean J   Chiron Raphaël R   Grenga Lucia L  

Frontiers in microbiology 20221013


Microorganisms proteotyping by tandem mass spectrometry has been recently shown as a powerful methodology to identify the wide-range taxonomy and biomass of microbiota. Sputum is the recommended specimen for routine microbiological monitoring of Cystic Fibrosis (<i>CF</i>) patients but has been rarely submitted to tandem mass spectrometry-based proteotyping. In this study, we compared the microbial components of spontaneous and induced sputum samples from three cystic fibrosis patients. Although  ...[more]

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