Proteomics

Dataset Information

0

Npl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA

ABSTRACT: Assessing the function of the RNA-binding activity of RNA-binding proteins (RBPs) is challenging. Here, Keil et al. use a targeted XL-ms approach to identify RNA-binding sites in components of nuclear mRNPs in S. cerevisiae. The analysis of RNA-binding mutants of one selected example, the SR-like protein Npl3, uncovers two novel functions of Npl3, namely in the recruitment of mRNP components to transcribed protein-coding genes and their transfer onto the mRNA. This data set applies label-free protein quantification to assess protein abundancies in NPL3 or CBC2 affinity purification experiments from cellular lysates of NPL3-WT and NPL3-P196D/A197D double-mutant expressing cells.

INSTRUMENT(S): Orbitrap Fusion, Q Exactive HF

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Henning Urlaub  

LAB HEAD: Prof. Dr. Henning Urlaub

PROVIDER: PXD034656 | Pride | 2023-03-11

REPOSITORIES: Pride

Json Xml
altmetric image

Publications

Npl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA.

Keil Philipp P   Wulf Alexander A   Kachariya Nitin N   Reuscher Samira S   Hühn Kristin K   Silbern Ivan I   Altmüller Janine J   Keller Mario M   Stehle Ralf R   Zarnack Kathi K   Sattler Michael M   Urlaub Henning H   Sträßer Katja K  

Nucleic acids research 20230101 2

RNA-binding proteins (RBPs) control every RNA metabolic process by multiple protein-RNA and protein-protein interactions. Their roles have largely been analyzed by crude mutations, which abrogate multiple functions at once and likely impact the structural integrity of the large ribonucleoprotein particles (RNPs) these proteins function in. Using UV-induced RNA-protein crosslinking of entire cells, protein complex purification and mass spectrometric analysis, we identified >100 in vivo RNA crossl  ...[more]

Similar Datasets

Transcriptomics The SR-like protein Npl3 transfers mRNP components from the transcription site onto the mRNA
2022-11-26 | GSE160709 | GEO
Proteomics Npl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA
2023-03-11 | PXD035153 | Pride
Proteomics Npl3 stabilizes R-loops at telomeres to prevent accelerated replicative senescence
2020-01-31 | PXD016772 | Pride
Transcriptomics Unphosphorylated SR-like Protein Npl3 Stimulates RNA Polymerase II Elongation
2008-09-06 | GSE12677 | GEO
Transcriptomics Loss of the yeast SR protein Npl3 alters gene expression due to transcription readthrough
2015-12-03 | GSE70191 | GEO
Transcriptomics A single SR-like protein, Npl3, promotes pre-mRNA splicing in budding yeast
2008-12-31 | GSE11491 | GEO
Transcriptomics Loss of the yeast SR protein Npl3 alters gene expression due to transcription readthrough
2015-12-02 | E-MTAB-3642 | biostudies-arrayexpress
Transcriptomics The structure and target identification of the tandem RRM of yeast Npl3 bound to RNA reveal a cooperative sequence-specific recognition and an RNA chaperone role in splicing
2022-12-01 | E-MTAB-11736 | biostudies-arrayexpress
Genomics ChIP-chip data of Npl3-Myc and Rrm3-Flag in wild-type and npl3∆ cells
2013-11-15 | GSE50185 | GEO
Genomics Metamycoplasma hyosynoviae strain:NPL3
| PRJNA238375 | ENA