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LC-MS/MS Proteomics analysis of LncRNA SNHG6 in HEPG2 cell line

ABSTRACT: To identify the proteins interact with LncRNA SNHG6 in human cell lines, we analyzed the protein using RNA-Pulldown followed by mass spectrometry in HEPG2. We identified proteins associated with the SNHG6 sense/anti-sense in the isolated complexes using MS and searched the human databases for these proteins.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Cell Culture

DISEASE(S): Liver Cancer

SUBMITTER: Aifu Lin

LAB HEAD: Aifu Lin

PROVIDER: PXD034782 | Pride | 2022-10-14

REPOSITORIES: Pride

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Long non-coding RNA SNHG6 couples cholesterol sensing with mTORC1 activation in hepatocellular carcinoma.

Liu Fangzhou F Tian Tian T Zhang Zhen Z Xie Shanshan S Yang Jiecheng J Zhu Linyu L Wang Wen W Shi Chengyu C Sang Lingjie L Guo Kaiqiang K Yang Zuozhen Z Qu Lei L Liu Xiangrui X Liu Jian J Yan Qingfeng Q Ju Huai-Qiang HQ Wang Wenqi W Piao Hai-Long HL Shao Jianzhong J Zhou Tianhua T Lin Aifu A

Nature metabolism 20220822 8

Cholesterol contributes to the structural basis of biological membranes and functions as a signaling molecule, whose dysregulation has been associated with various human diseases. Here, we report that the long non-coding RNA (lncRNA) SNHG6 increases progression from non-alcoholic fatty liver disease (NAFLD) to hepatocellular carcinoma (HCC) by modulating cholesterol-induced mTORC1 activation. Mechanistically, cholesterol binds ER-anchored FAF2 protein to promote the formation of a SNHG6-FAF2-mTO ...[more]

PMID: 35995997

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