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HDX-MS Analysis of the protein ALT51

ABSTRACT: HDX-MS analysis of the protein ALT51, comparing deuterium uptake between the full length protein and a truncated construct missing the CBM-like domain

INSTRUMENT(S): impact HD

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: John Burke

LAB HEAD: Dr. John E. Burke

PROVIDER: PXD034850 | Pride | 2023-02-22

REPOSITORIES: Pride

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A previously uncharacterized O-glycopeptidase from Akkermansia muciniphila requires the Tn-antigen for cleavage of the peptide bond.

Medley Brendon J BJ Leclaire Leif L Thompson Nicole N Mahoney Keira E KE Pluvinage Benjamin B Parson Matthew A H MAH Burke John E JE Malaker Stacy S Wakarchuk Warren W Boraston Alisdair B AB

The Journal of biological chemistry 20220830 10

Akkermansia muciniphila is key member of the human gut microbiota that impacts many features of host health. A major characteristic of this bacterium is its interaction with host mucin, which is abundant in the gut environment, and its ability to metabolize mucin as a nutrient source. The machinery deployed by A. muciniphila to enable this interaction appears to be extensive and sophisticated, yet it is incompletely defined. The uncharacterized protein AMUC_1438 is encoded by a gene that was pre ...[more]

PMID: 36049519

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