Proteomics

Dataset Information

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Salt and metal tolerance involves formation of guttation droplets in species of the Aspergillus versicolor complex


ABSTRACT: Three strains of the Aspergillus versicolor complex were isolated from a salty marsh at a former uranium mining site in Thuringia, Germany. The strains from a metal-rich environment were not only highly salt tolerant (up to 20% NaCl), but at the same time could sustain elevated Cs and Sr (both up to 100 mM) concentrations as well as other (heavy) metals present in the environment. During growth experiments when screening for differential cell morphology, the occurrence of guttation droplets was observed, specifically when elevated Sr concentrations of 25 mM were present in the media. To analyze the potential of metal tolerance being promoted by these excretions, proteomics and metabolomics of guttation droplets were performed. Indeed, proteins involved in up-regulated metabolic activities as well as in stress responses were identified. The metabolome verified the presence of amino sugars, glucose homeostasis regulating substance, (S)-abscisic acid and bioactive alkaloids, flavones and quinones.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Aspergillus Puulaauensis

SUBMITTER: Thomas Krüger  

LAB HEAD: Axel A. Brakhage

PROVIDER: PXD035065 | Pride | 2022-09-12

REPOSITORIES: Pride

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