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Proteomic analysis of the secretome of selected Mycobacterium tuberculosis Erdman strains

ABSTRACT: Extracellular Mycobacterium tuberculosis (Mtb) aggregates can evade phagocytosis and intracellular host-cell defenses by inducing macrophage killing. We showed that Mtb aggregates require a functional ESX-1 type VII secretion system and production of the surface-exposed lipid phthiocerol dimycocerosate (PDIM) to induce cell death upon contact with macrophages. We used quantitative proteomics to compare the secretome of a panel of Mtb mutant strains that induce or do not induce macrophage death and identified secreted bacteria factors involved in macrophage death induction.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Bacteria Mycobacterium Tuberculosis Str. Erdman = Atcc 35801

DISEASE(S): Tuberculosis

SUBMITTER: Chiara Toniolo

LAB HEAD: John McKinney

PROVIDER: PXD035080 | Pride | 2023-03-16

REPOSITORIES: Pride

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