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Proteomic analysis of reticulocytes treated with the PIEZO-1 activator YODA

ABSTRACT: We performed a label free proteomic analysis on reticulocytes produced in vitro from CD34+ of healthy donors cells either exposed to DMSO as a control or to the PIEZO1 activator YODA1 at 1µM.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Blood

SUBMITTER: Virginie Salnot

LAB HEAD: Loïc Garçon

PROVIDER: PXD035122 | Pride | 2023-03-11

REPOSITORIES: Pride

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Red blood cell proteomics reveal remnant protein biosynthesis and folding pathways in PIEZO1-related hereditary xerocytosis.

Caulier Alexis A Jankovsky Nicolas N Gautier Emilie Fleur EF El Nemer Wassim W Guitton Corinne C Ouled-Haddou Hakim H Guillonneau François F Mayeux Patrick P Salnot Virginie V Bruce Johanna J Picard Véronique V Garçon Loïc L

Frontiers in physiology 20221201

Hereditary xerocytosis is a dominant red cell membrane disorder characterized by an increased leak of potassium from the inside to outside the red blood cell membrane, associated with loss of water leading to red cell dehydration and chronic hemolysis. 90% of cases are related to heterozygous gain of function mutations in PIEZO1, encoding a mechanotransductor that translates a mechanical stimulus into a biological signaling. Data are still required to understand better PIEZO1-HX pathophysiology. ...[more]

PMID: 36531183

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2018-09-18 | PXD009258 | Pride

Proteomic characterization of exosomes derived from malaria-infected reticulocytes, in a murine malaria model

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Glyoxal induces senescence of human keratinocytes through oxidative stress and activation of the AKT/FOXO3a/p27KIP1 pathway

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