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Plasma membrane analysis of NAA60-deficient mouse mammary tumor cells

ABSTRACT: Analysis of plasma membrane enriched protein fractions from NAA60-proficient or deficient mouse mammary tumor cells.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Breast Cancer Cell Line

SUBMITTER: Manfred Heller

LAB HEAD: Sven Rottenberg

PROVIDER: PXD035143 | Pride | 2025-08-21

REPOSITORIES: Pride

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			Action	DRS
	20210119_WCL12_KO3_CD_i01.raw	Raw
	20210119_WCL12_KO3_CD_i02.raw	Raw
	20210119_WCL18_KO9_CD_i01.raw	Raw
	20210119_WCL18_KO9_CD_i02.raw	Raw
	20210119_WCL2_WT2_CD_i01.raw	Raw

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Publications

NAA60 facilitates LRRC8A- and LRRC8D-mediated platinum drug uptake.

Widmer Carmen Alexandra CA Moyseos Anna A Klebic Ismar I Dettwiler Martina M González-Fernández Martín M Gogola Ewa E Siffert Myriam M Buchs Natasha N Braga-Lagache Sophie S Uldry Anne-Christine AC Jonkers Jos J Heller Manfred M Rottenberg Sven S

Communications biology 20251006 1

The platinum-based drugs cis- and carboplatin, which are crucial for treating cancers with DNA repair defects, like those caused by BRCA1/2 mutations, rely on the volume-regulated anion channel subunits LRRC8A and LRRC8D for about 50% of cellular drug uptake. Yet, the precise mechanisms of how LRRC8A and LRRC8D mediate this function are largely unknown. Here, we identify NAA60, an N-terminal acetyltransferase, which localizes to the Golgi apparatus to affect LRRC8A and LRRC8D function. Our data ...[more]

PMID: 41053424

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Mass spectrometry identification of partners of the Toxoplasma gondii HCF101 protein

Project description:Toxoplasma gondii is a parasitic protist that is the agent of toxoplasmosis. It is capable of infecting a wide variety of vertebrates, including humans. The infection is mainly asymptomatic in immunocompetent patients, but in case of immunosuppression or for the congenital form of toxoplasmosis it can lead to severe pathologies with a possible fatal outcome. Like for other eukaryotes, many key cellular functions in T. gondii involve proteins containing an iron-sulfur cluster as a cofactor. Cytosolic and nuclear iron-sulfur proteins depend on a specific pathway for assembling their iron-sulfur cofactor. We have investigated the T. gondii homolog of the HCF101 protein, initially characterized in plants as a chloroplast-based iron-sulfur transfer protein, by co-immunoprecipitating associated protein partners and identifying them by mass spectrometry. It confirmed that T. gondii HCF101 is not involved in plastid-based iron-sulfur metabolism, but in the biogenesis of cytosolic and nuclear iron-sulfur proteins instead.

2025-01-24 | PXD051551 | Pride

Transcription factor Ebf1 regulates differentiation stage-specific signaling, proliferative expansion and survival of B cells [EBF1]

Project description:Transcription factor Ebf1 is an important determinant of early B lymphopoiesis. To gain insight into differentiation stage-specific functions of Ebf1, we conditionally inactivated Ebf1. We found that Ebf1 is required for proliferation, survival and signaling of pro-B cells and peripheral B cell subsets. The proliferation defect of Ebf1-deficient pro-B cells, including the impaired expression of IL-7Ra and several cell cycle regulators, is overcome by transformation with v-Abl. The survival defect of transformed Ebf1fl/fl pro-B cells can be rescued by the forced expression of the Ebf1 targets c-Myb or Bcl-xL. In mature B cells, Ebf1 deficiency interferes with the BAFF-R and BCR-dependent Akt signaling pathways, as well as with germinal center formation and class switch recombination. Genome-wide analyses of Ebf1 binding and Ebf1-mediated gene expression in mature B cells and comparison with reported data sets in pro-B cells provide insight into the basis for lineage- and stage-specific functions of Ebf1. EBF1 binding in splenic B cells in mice

2012-04-02 | E-GEOD-35910 | biostudies-arrayexpress

Multi-omics analysis reveals mechanisms of complete resistance to extreme illumination levels in a green alga isolated from desert soil crusts

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KINETIC AND COMPOSITIONAL ANALYSIS OF RNA POLYMERASE II IN TRYPANOSOMA BRUCEI

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2023-05-23 | PXD039878 | Pride

Dynamic 23S rRNA modification ho5C2501 benefits Escherichia coli under oxidative stress

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2022-02-16 | PXD025875 | Pride

Chaperone-mediated autophagy promotes PCa survival during ARPI through selective proteome remodeling

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2023-01-26 | PXD036227 | Pride

Transcription factor Ebf1 regulates differentiation stage-specific signaling, proliferative expansion and survival of B cells

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2012-04-02 | E-GEOD-35915 | biostudies-arrayexpress

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