Proteomics

Dataset Information

0

Natural aging and ovariectomy induces parallel phosphoproteomic alterations in skeletal muscle of female mice


ABSTRACT: The loss of skeletal muscle strength mid-life in females is associated with the decline of estrogen. Here, we questioned how estrogen deficiency might impact the overall skeletal muscle phosphoproteome after contraction, as force production induces phosphorylation of several muscle proteins. Phosphoproteomic analyses of the tibialis anterior muscle after contraction in two mouse models of estrogen deficiency, ovariectomy (Ovariectomized [Ovx] vs Sham) and natural aging-induced ovarian senescence (Older Adult [OA] vs Young Adult [YA]), identified a total of 2,593 and 3,507 phosphopeptides in Ovx/Sham and OA/YA datasets, respectively. Further analysis of estrogen deficiency-associated proteins and phosphosites identified 66 proteins and 21 phosphosites from both datasets. Of these, 4 estrogen deficiency-associated proteins and 4 estrogen deficiency-associated phosphosites were significant and differentially phosphorylated or regulated, respectively. Comparative analyses between Ovx/Sham and OA/YA using Ingenuity Pathway Analysis (IPA) found parallel patterns of inhibition and activation across IPA-defined canonical signaling pathways and physiological functional analysis, which were similarly observed in downstream GO, KEGG, and Reactome pathway overrepresentation analysis pertaining to muscle structural integrity and contraction, including AMPK and calcium signaling. IPA Upstream regulator analysis identified MAPK1 and PRKACA as candidate kinases and calcineurin as a candidate phosphatase sensitive to estrogen. Our findings highlight key molecular signatures and pathways in contracted muscle suggesting that the similarities identified across both datasets could elucidate molecular mechanisms that may contribute to skeletal muscle strength loss due to estrogen deficiency.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Mina Peyton  

LAB HEAD: Dawn Lowe

PROVIDER: PXD035171 | Pride | 2023-09-01

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
17855_LFQ_M01.raw Raw
17855_LFQ_M02.raw Raw
17855_LFQ_M03.raw Raw
17855_LFQ_M04.raw Raw
17855_LFQ_M06.raw Raw
Items per page:
1 - 5 of 19

Similar Datasets

2022-09-28 | PXD035107 | Pride
2022-12-05 | GSE194075 | GEO
2011-07-22 | E-GEOD-30851 | biostudies-arrayexpress
2021-11-17 | GSE184493 | GEO
2011-07-23 | GSE30851 | GEO
2022-07-07 | GSE197085 | GEO
2022-10-25 | PXD037316 | Pride
2021-08-16 | GSE157396 | GEO
2009-05-20 | E-GEOD-11622 | biostudies-arrayexpress
2012-11-15 | E-GEOD-42295 | biostudies-arrayexpress