Dataset Information

Proteomic profiling of carotid atherosclerotic plaques and adjacent intact arterial segments

ABSTRACT: Although it is well known that the extracellular matrix composition significantly impacts the development of neointimal hyperplasia and atherosclerosis, the biochemical cues regulating the cell-ECM interactions within the neointima remain obscure. Here, we pairwise collected 14 atherosclerotic plaques excised during the carotid endarterectomy and 14 adjacent intact aortic segments and then interrogated their proteomic profile.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Atherosclerotic Plaque

DISEASE(S): Atherosclerosis

SUBMITTER: Arseniy Lobov

LAB HEAD: Anton G. Kutikhin

PROVIDER: PXD035173 | Pride | 2025-06-05

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	62_Slot1-54_1_2674.d.zip	Other
	62_Slot1-54_1_2714.d.zip	Other
	63_Slot1-42_1_2676.d.zip	Other
	63_Slot1-42_1_2716.d.zip	Other
	64_Slot1-38_1_2678.d.zip	Other

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Publications

Citrullination Accompanies the Development of Carotid Atherosclerotic Plaques.

Kanonykina Anastasia A Velikanova Elena E Markova Victoria V Bogdanov Leo L Shishkova Daria D Shabaev Amin A Sinitsky Maxim M Sinitskaya Anna A Poddubnyak Alyona A Lazebnaya Anastasia A Stepanov Alexander A Tyurina Arina A Lobov Arseniy A Zainullina Bozhana B Yuzhalin Arseniy A Kutikhin Anton A

Current medicinal chemistry 20241017

<h4>Background</h4>Citrullination represents a post-translational modification primarily mediated by peptidylarginine deiminase (PADI) 2 and 4 and resulting in the conversion of positively charged peptidylarginine to neutrally charged peptidylcitrulline. Molecular consequences of citrullination include the generation of neoepitopes which provoke the production of autoantibodies implicated in the development of autoimmune diseases. As citrullination initiates, promotes, and is enhanced by aseptic ...[more]

PMID: 39421992

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Project description:Oral transmission of T. cruzi is probably the most frequent mechanism among animals in the wild. In this context, there is a high prevalence of human infections in regions where triatomine infection with T. cruzi is low. This led to the hypothesis that the consumption of raw or undercooked meat from animals infected with T. cruzi could be responsible for the transmission of the infection. Therefore, the general objective of this study was to demonstrate the role of meat consumption from infected animals in the oral transmission of T. cruzi infection. Groups of five female mice Balb/c were fed with muscles obtained from mice in the acute phase of infection by the clone H510 C8C3hvir of T. cruzi, and the infection of the fed mice was monitored by a parasitemia curve. Similarly, we assessed the infective capacity of T. cruzi trypomastigotes and amastigotes by infecting groups of five mice Balb/c females, which were infected orally using a nasogastric probe, and the infection was monitored by parasitemia curve. Finally, different trypomastigote and amastigote inoculums were used to determine their infective capacity. Adhesion assays of T. cruzi proteins to AGS stomach cells were performed and the adhered proteins were detected by western blotting using monoclonal or polyclonal antibodies. In all cases, 60–100% of animals were fed meat from mice infected in the acute phase or infected by means of a nasogastric probe with trypomastigotes or amastigotes. These animals developed high parasitemia, and 80% died around day 40 post-infection. The adhesion tests showed that cruzipain is a molecule of trypomastigotes and amastigotes that binds to AGS cells. LC-MS/MS also confirmed that transialidase may be involved in TCT attachment of TCT or invasion of stomach cells. It was concluded that the consumption of meat from infected animals in the acute phase of T. cruzi infection allows transmission of the infection. Similarly, trypomastigotes and amastigotes were able to infect mice when administered orally, while cruzipain was a relevant molecule in this infective process.