Proteomics

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Monoclonal antibody affinity-enrichment pull-down proteomics for Fasciola hepatica gut-associated proteins

ABSTRACT: The zoonotic liver fluke Fasciola hepatica is responsible for economic losses to the agriculture industry worldwide. Triclabendazole has been the drug of choice. However, anthelmintic resistance is becoming prevalent with cases of resistance detected globally. This emphasizes the need for alternative control methods. A significant focus is on the development of protein vaccines. In this process however, glycoproteins (GPs) have been mostly ignored. GPs are present throughout the organ systems of F. hepatica and such gut-associated GPs could serve as ‘hidden’ antigens. Hidden antigens are not initially exposed to the host’s immune system during the course of natural infections, but have proven to be effective vaccine candidates, notably for antigens that are present in the intestine of blood-feeding and tissue-dwelling parasites. This work describes the purification of gut-associated parasite GPs based on protective antibodies against adult flukes in vitro, which demonstrate a binding on fluke gut structures. Finally, we provide a list of promising novel F. hepatica gut-associated vaccine candidates.

INSTRUMENT(S):

ORGANISM(S): Fasciola Hepatica (liver Fluke)

SUBMITTER: Ramon Eichenberger  

LAB HEAD: Ramon Marc Eichenberger

PROVIDER: PXD035194 | Pride | 2026-06-29

REPOSITORIES: Pride

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20210504_002_S296877_PD001_mAb324.raw Raw
20210504_003_S296881_PD005_mAb411.raw Raw
20210504_004_S296884_PD008_crude.raw Raw
20210504_005_S296883_PD007_crude.raw Raw
20210504_007_S296878_PD002_mAb324.raw Raw
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Publications

Identification of <i>Fasciola hepatica</i> gut-associated glycoproteins as potential vaccine candidates by lectin-affinity chromatography, flukicidal monoclonal antibodies, and affinity-enrichment mass spectrometry.

Rechsteiner Sonja G SG   Hasler Sina S   Gehrig Peter P   Deplazes Peter P   Eichenberger Ramon M RM  

Frontiers in parasitology 20260529

A significant economic impact to the agriculture and declining drug efficacy due to anthelmintic resistance lead to the search for novel intervention approaches against the zoonotic liver fluke <i>Fasciola hepatica</i>. A cost-effective strategy is to induce protective immunity by vaccination. However, adaptation of the parasite to evade the host immune response hampers classical vaccine development. Therefore, an ideal protective epitope would not be subjected to this evolutionary selection. An  ...[more]

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