Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive HF
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Keratinocyte
DISEASE(S): Disease Free
SUBMITTER: Timur Shkrigunov
LAB HEAD: Natalia Petushkova
PROVIDER: PXD035202 | Pride | 2022-07-20
REPOSITORIES: Pride
Current issues in molecular biology 20220509 5
An important step in the proteomic analysis of missing proteins is the use of a wide range of tissues, optimal extraction, and the processing of protein material in order to ensure the highest sensitivity in downstream protein detection. This work describes a purification protocol for identifying low-abundance proteins in human chorionic villi using the proposed "1DE-gel concentration" method. This involves the removal of SDS in a short electrophoresis run in a stacking gel without protein separ ...[more]