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NUPR1 inhibits hyperPARylation-associated cell death

ABSTRACT: Nuclear Protein 1 (NUPR1) is a nuclear intrinsically disordered protein of 82 amino acids that plays an important role in stress response and in cancer development. The project was aimed at identifying binding partners of NUPR1 in MiaPaCa-2 cells, in control and after stress condition: glucose starvation during 24h. Lysates from MiaPaCa-2 cells (transfected with NUPR1-Flag or NUPR1-GFP) were used for immunoprecipitation, performed with agarose beads coated with anti-Flag or anti-GFP antibodies. After immunoprecipitation, the eluted proteins were analyzed by LC-MS/MS.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Pancreas

DISEASE(S): Pancreatic Carcinoma

SUBMITTER: AUDEBERT Stephane

LAB HEAD: Dr. Santofimia Patricia

PROVIDER: PXD035236 | Pride | 2022-07-29

REPOSITORIES: Pride

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NUPR1 protects against hyperPARylation-dependent cell death.

Santofimia-Castaño Patricia P Huang Can C Liu Xi X Xia Yi Y Audebert Stephane S Camoin Luc L Peng Ling L Lomberk Gwen G Urrutia Raul R Soubeyran Philippe P Neira Jose Luis JL Iovanna Juan J

Communications biology 20220722 1

Proteomic, cellular and biochemical analysis of the stress protein NUPR1 reveals that it binds to PARP1 into the nucleus and inhibits PARP1 activity in vitro. Mutations on residues Ala33 or Thr68 of NUPR1 or treatment with its inhibitor ZZW-115 inhibits this effect. PARylation induced by 5-fluorouracil (5-FU) treatment is strongly enhanced by ZZW-115 and associated with a decrease of NAD<sup>+</sup>/NADH ratio and rescued by the PARP inhibitor olaparib. Cell death induced by ZZW-115 treatment of ...[more]

PMID: 35869257

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