In vivo polycystin-1 interactome using heads of Pkd1 knock-in newborn mice

ABSTRACT: We generated a new mouse model with three copies of the HA epitope and eGFP knocked-in frame into the endogenous mouse Pkd1 gene by CRISPR/Cas9. The modified allele is fully functional. Using nanobody-coupled beads and large quantities of tissue (8 heads of P1 newborn mice / experiment / condition), we immunoprecipitated a PC1 interactome that was characterized by mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

DISEASE(S): Polycystic Kidney Disease

SUBMITTER: David Anderson  

LAB HEAD: Gregory George Germino

PROVIDER: PXD035250 | Pride | 2023-08-08

REPOSITORIES: Pride