Proteomics

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MTORC2 interactome and localization determine aggressiveness of high-grade glioma cells through association with gelsolin.

ABSTRACT: The mTOR complex 2 (mTORC2) has been implicated as a key regulator of glioblastoma cell migration. However, the roles of mTORC2 in the migrational control process have not been entirely elucidated. Here we elaborate that mTORC2 is crucial for GBM cell motility. Inhibition of mTORC2 resulted in impaired cell movement, affecting microfilaments and microtubules' functions. Later, we quantitatively characterized the mTORC2 interactome using affinity-purification mass spectrometry (AP-MS) in glioblastoma. We demonstrated that changes in cell migration ability alter mTORC2-associated proteins. These interacting proteins help determine the capability of glioma cell movement.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

DISEASE(S): Brain Glioblastoma Multiforme,Brain Cancer

SUBMITTER: Naphat Chantaravisoot  

LAB HEAD: Trairak Pisitkun

PROVIDER: PXD035256 | Pride | 2023-05-10

REPOSITORIES: Pride

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mTORC2 interactome and localization determine aggressiveness of high-grade glioma cells through association with gelsolin.

Chantaravisoot Naphat N   Wongkongkathep Piriya P   Kalpongnukul Nuttiya N   Pacharakullanon Narawit N   Kaewsapsak Pornchai P   Ariyachet Chaiyaboot C   Loo Joseph A JA   Tamanoi Fuyuhiko F   Pisitkun Trairak T  

Scientific reports 20230429 1

mTOR complex 2 (mTORC2) has been implicated as a key regulator of glioblastoma cell migration. However, the roles of mTORC2 in the migrational control process have not been entirely elucidated. Here, we elaborate that active mTORC2 is crucial for GBM cell motility. Inhibition of mTORC2 impaired cell movement and negatively affected microfilament and microtubule functions. We also aimed to characterize important players involved in the regulation of cell migration and other mTORC2-mediated cellul  ...[more]

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