Dataset Information

Identification of Key Events and Regulatory Networks in the Formation Process of Primordial Germ Cell based on Proteomics

ABSTRACT: Studies have analyzed the regulation mechanism of primordial germ cell (PGC) formation at the transcriptional level, but few at the protein level, showing the mechanism analysis of PGC formation not systematic. We collected embryonic stem cells and PGC using a novel sampling strategy and performed label-free proteomics to screen for differential expression proteins (DEPs) in PGC formation.

INSTRUMENT(S):

ORGANISM(S): Gallus Gallus (chicken)

TISSUE(S): Primary Cell

SUBMITTER: Qisheng Zuo

LAB HEAD: Qisheng Zuo

PROVIDER: PXD035490 | Pride | 2025-09-08

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	S9421LQ_ESC_1_Slot2-51_1_2800.d.rar	Other
	S9421LQ_ESC_2_Slot2-52_1_2802.d.rar	Other
	S9421LQ_PGC_1_Slot2-49_1_2796.d.rar	Other
	S9421LQ_PGC_2_Slot2-50_1_2798.d.rar	Other
	checksum.txt	Txt

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Publications

Ubiquitination plays an important role during the formation of chicken primordial germ cells.

Gong Wei W Liu Xin X Lv Xiaoqian X Zhang Yani Y Niu Yingjie Y Jin Kai K Li Bichun B Zuo Qisheng Q

Journal of animal science 20240101

As an important posttranslational modification, ubiquitination plays an important role in regulating protein homeostasis in eukaryotic cells. In our previous studies, both the transcriptome and proteome suggested that ubiquitination is involved in the formation of chicken primordial germ cells (PGCs). Here, affinity enrichment combined with liquid chromatography-tandem mass spectrometry (MS/MS) was used to analyze the ubiquitome during the differentiation from embryonic stem cells to PGCs, and w ...[more]

PMID: 39187982

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