Proteomic and phosphoproteomic profiling reveals the oncogenic role of protein kinase D family kinases in cholangiocarcinoma

ABSTRACT: To systematically characterize the kinome of the CCA, the proteomic and phosphoproteomic profiling of CCA tumors with NATs was performed using LC-MS/MS (Figure 1). After the extraction and digestion of proteins from each sample, peptides were labeled with TMT reagent and fractionated, and phosphopeptides were enriched with immobilized metal ion affinity chromatography (IMAC).

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Bile Duct

DISEASE(S): Cholangiocarcinoma

SUBMITTER: yun lu  

LAB HEAD: Biliary and Pancreatic Surgery/Cancer Research Center

PROVIDER: PXD035652 | Pride | 2022-10-18

REPOSITORIES: Pride