Dataset Information

Proteomic and phosphoproteomic profiling reveals the oncogenic role of protein kinase D family kinases in cholangiocarcinoma

ABSTRACT: To systematically characterize the kinome of the CCA, the proteomic and phosphoproteomic profiling of CCA tumors with NATs was performed using LC-MS/MS (Figure 1). After the extraction and digestion of proteins from each sample, peptides were labeled with TMT reagent and fractionated, and phosphopeptides were enriched with immobilized metal ion affinity chromatography (IMAC).

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Bile Duct

DISEASE(S): Cholangiocarcinoma

SUBMITTER: yun lu

LAB HEAD: Biliary and Pancreatic Surgery/Cancer Research Center

PROVIDER: PXD035652 | Pride | 2022-10-18

REPOSITORIES: Pride

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Proteomic and Phosphoproteomic Profiling Reveals the Oncogenic Role of Protein Kinase D Family Kinases in Cholangiocarcinoma.

Lu Yun Y Li Xiangyu X Zhao Kai K Shi Yuanxin Y Deng Zhengdong Z Yao Wei W Wang Jianming J

Cells 20220930 19

Cholangiocarcinoma (CCA) is a lethal malignancy in the hepatobiliary system, with dysregulated protein expression and phosphorylation signaling. However, the protein and phosphorylation signatures of CCAs are little-known. Here, we performed the proteomic and phosphoproteomic profiling of tumors and normal adjacent tissues (NATs) from patients with CCA and predicted eleven PKs high-potentially related to CCA with a comprehensive inference of the functional protein kinases (PKs) (CifPK) pipeline. ...[more]

PMID: 36231050

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