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Identification of regenerating island-derived protein 3E in dogs

ABSTRACT: Our aim was to identify Regenerating islet-derived (REG proteins in dogs. We annotated REG-family members in the canine genome and proteome, and identified one member of the REG3 subfamily, consequently termed REG3E by the Vertebrate Gene Nomenclature Committee. We verified REG3E gene expression using publicly available RNA-seq data (www.barkbase.org). We investigated the presence of REG3E protein in canine pancreatic tissue and plasma with Western blot and immunohistochemistry, using anti-human REG3A antibodies. Protein identity was subsequently confirmed with mass spectrometry on canine pancreas homogenates and plasma.

INSTRUMENT(S):

ORGANISM(S): Canis Familiaris (dog) (canis Lupus Familiaris)

TISSUE(S): Blood Plasma, Pancreas

SUBMITTER: Manfred Heller

LAB HEAD: Laureen Peters

PROVIDER: PXD035694 | Pride | 2022-10-17

REPOSITORIES: Pride

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			Action	DRS
	20210308_1_BeRi_41963306_LP_i01.raw	Raw
	20210308_1_BeRi_41963306_LP_i02.raw	Raw
	20210308_LP_pepxml.tar.gz	Other
	20210818_Dog_Prancreas_LP_i01.raw	Raw
	20210818_LP_pepxml.tar.gz	Other

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