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Proteomic analysis of the extracellular vesicles of the filamentous fungus Botrytis cinerea.

ABSTRACT: Botrytis cinerea is the causing agent of grey mould on hundreds of plants cultivated in fields, tunnels and greenhouses worldwide. Considering the central role of secretion in the necrotrophic interaction between the fungus and its host plants, non conventional secre-tion was explored through the search and characterisation of extracellular vesicles. In or-der to reveal the protein composition of these vesicles, a proteomic analysis was chosen.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Botryotinia Fuckeliana (noble Rot Fungus) (botrytis Cinerea)

TISSUE(S): Extracellular Vesicle

SUBMITTER: Dupuy Jean-William

LAB HEAD: Christophe Bruel

PROVIDER: PXD035766 | Pride | 2023-05-02

REPOSITORIES: Pride

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Extracellular Vesicles of the Plant Pathogen <i>Botrytis cinerea</i>.

De Vallée Amelie A Dupuy Jean-William JW Moriscot Christine C Gallet Benoit B Vanderperre Solène S Guignard Gaëtan G Rascle Christine C Calvar Glen G Malbert Bastien B Gillet François-Xavier FX Dieryckx Cindy C Choquer Mathias M Girard Vincent V Poussereau Nathalie N Bruel Christophe C

Journal of fungi (Basel, Switzerland) 20230420 4

Fungal secretomes are known to contain a multitude of components involved in nutrition, cell growth or biotic interactions. Recently, extra-cellular vesicles have been identified in a few fungal species. Here, we used a multidisciplinary approach to identify and characterize extracellular vesicles produced by the plant necrotroph <i>Botrytis cinerea</i>. Transmission electron microscopy of infectious hyphae and hyphae grown in vitro revealed extracellular vesicles of various sizes and densities. ...[more]

PMID: 37108947

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