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Concentration and composition of the protein corona as a function of incubation time and serum concentration

ABSTRACT: We describe the concentration and composition of the protein corona as a function of incubation time (30 min and 1 day) and the concentration of fetal bovine serum (FBS) used to form the corona (5%, 10%, and 100%) on 200 nm carboxylate-modified magnetic NPs, using semi-automated processes.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Blood Serum

SUBMITTER: Karsten Poulsen

LAB HEAD: Christine Payne

PROVIDER: PXD035811 | Pride | 2023-03-21

REPOSITORIES: Pride

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Concentration and composition of the protein corona as a function of incubation time and serum concentration: an automated approach to the protein corona.

Poulsen Karsten M KM Payne Christine K CK

Analytical and bioanalytical chemistry 20220826 24

Nanoparticles in contact with proteins form a "corona" of proteins adsorbed on the nanoparticle surface. Subsequent biological responses are then mediated by the adsorbed proteins rather than the bare nanoparticles. The use of nanoparticles as nanomedicines and biosensors would be greatly improved if researchers were able to predict which specific proteins will adsorb on a nanoparticle surface. We use a recently developed automated workflow with a liquid handling robot and low-cost proteomics to ...[more]

PMID: 36018335

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